Fundamentals of Microbiology

Practical

Introduction to microscopy; study of different types of microscopes; wet mount and hanging drop preparations; sterilization by different methods, filteration, dry heat, moist heat, chemical use etc. staining techniques-simple, differential, structural and microchemical techniques; distribution of micro-organisms in nature; isolation, pure culture and preservation methods; enumeration of micro-organisms, environmental influences on micro-organisms; biochemical tests; observation of fungi, blue-green algae, bacteria and protozoans. Study and enumeration of coliform, iron, and sulphur bacteria, viable nonculturable bacteria. Microbiological water quality management in aquaculture, bioremediators and probiotics. Pathogens of aquatic animals - their isolation and identifications; detection of toxic organisms in aquatic system.