2.1.2 Bright field microscope

2.1.2 Bright field microscope

Ordinary compound microscope is called a bright – field microscope, because it forms a dark image against a brighter background.

Image formation is shown in the figure (the path of light is shown)

Bright field microscope

The objective lens forms an enlarged real image within the microscope and the eyepiece (ocular) lens further magnifies this primary image into the final virtual image (which appears to lie about 25 cm away beyond the stage.

The total magnification is calculated by multiplying the objective and eyepiece magnification together. (For example, if 45x objective lens is used with a 10x ocular, the magnification of the specimen will be 450x.)

Last modified: Saturday, 31 December 2011, 5:23 AM