VPB 112: General Veterinary Biochemistry (1+1)

PREPARATION OF 1000 ml OF STANDARD N/10 Na₂CO₃ SOLUTION

• Na₂CO₃ is used as primary standard for alkali because it is obtained in pure state and weighed accurately.                                                             

                   Equivalent weight of Na₂CO₃ = ((23 x 2 + 12 + 16 x 3 ) / 2 )= 53 g

• So to prepare 1000 ml of 1 N Na₂CO₃ solution, amount of Na₂CO₃ required will be 53 g and for 1000 ml of 0.1 N Na₂CO₃ solution the amount of Na₂CO₃ required will be 5.3 g

Procedure

• Weigh exactly 5.3 g of Na₂CO₃.
• Then transfer this Na₂CO₃ to a beaker containing about 800 ml of distilled water.
• Dissolve the Na₂CO₃ completely by stirring.
• Add more distilled water and make up the volume to 1000 ml.