Neuromuscular, Digestive and Respiratory Systems: Using the Physiograph

Using the Physiograph

USING THE PHYSIOGRAPH

Put the recording paper in the paper rack and bring it to the top of the console.
Lift the pens using pen lift knob. Slide the paper under the pulley guides and under the ball bearing after lifiting it using thumbscrew.
Fill ink in the inkwells and check the flow of ink by pressing the inkwell lid and closing the opening; ink should flow freely.
Keep the pens lifted from the paper when not doing any recording.
Switch off the mains on the console and insert the desired coupler. (Coupler should not be inserted or taken out of the coupler housing while the switch is in ON position).
Connect the transducer with the coupler and with the experimental tissue or subject.
Put the main switch of the console in ON position, adjust the sensitivity to the desired level (Varies with different experiments).
Adjust the position of the pen to the centre. If required, connect the stimulator to physiograph, select a type of stimulus (single, double etc), adjust the voltage of the stimulus.
Carry out the muscle nerve preparation. Fix the femur with muscle grip and gently lay the nerve on the electrode without any tension.
The free end of the thread from the tendon is attached to the lever of the isotonic fine movements transducer to convert the mechanical activity into electrical activity.
Connect the transducer to the strain gauge coupler in the base unit. Stimulate the muscle with stimulator to provide single stimulus, successive stimuli and repetitive stimuil and record the specific contractions in the moving graph sheet.
For EKG recording, connect the electrodes of leads to the 5-pin junction box and then to the coupler.
Fix the electrodes of ECG to their respective place on the body after applying conductive jelly.
Include the bio-potential / EKG coupler to the base unit. Adjust sensitivity to 1 mV.
Keep the lead selector of the coupler to CAL position, run the paper and press the push button on the coupler 3 to 4 times while adjusting CAL control so that 1 mV produces 1 cm deflection of the pen.
Record the electrical activity of the heart.
For recording EEG and EMG from the skin surface or from muscle, small cup electrodes are used. A thick paste is used to keep the electrode in position.
Needle electrodes are used to record muscle action potential.

Last modified: Monday, 6 June 2011, 10:49 AM

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VPB 121: Veterinary Physiology - II (2+1)

Using the Physiograph

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