VPB 121: Veterinary Physiology - II (2+1)

BACTERIAL COUNT - PROCEDURE

• Prepare Strained Rumen Liquor (SRL) by filtering the rumen liquor through four layers of muslin cloth.
• Draw this SRL up to mark 0.5 in the R.B.C pipette.
• Carefully draw crystal violet stain solution up to 101 mark without any air bubbles.
• Mix the SRL sample and stain solution by rotating the RBC pipette horizontally between the two palms.
• Discard 3 or 4 drops of the unmixed stain solution from the stem of the pipette.
• Focus Neubaur counting chamber covered with cover slip under high power.
• Charge the diluted SRL between the counting chamber and the cover slip.
• Keep it undisturbed for few minutes. Count the number of bacteria in five medium squares like R.B.C. counting.