VMD 512: Zoo/Wild Animal Breeding, Management, Nutrition and Health Care (1+1)

SAMPLING AND EXAMINATION OF SAMPLES

Sampling

• Ear veins may be used for the collection of blood sample.
• Blood smears may be prepared from the blood sample obtained.
• Dung sample has to be collected freshly.
• Parasites may be collected if any on the body.
• Swab from any fresh wound or abscess
• Exudate from wound
• Discharge from trunk for evidence of diseases.

Examination of samples

Samples	Parameters to be examined
Dung	To find the evidence of bleeding, fauna, abnormal consistency, abnormal odour, sand materials, evidence of parasitic fauna like strongyles etc.
Peripheral blood smear	To rule out the differential leukocyte count and evidence of haemoprotozoa
Blood in EDTA	For haematological investigations like hematocrit value, hemoglobin, RBC, WBC, ESR, clotting time, Mean Corpuscular Volume, Mean Corpuscular Hemoglobin and Mean Corpuscular Hemoglobin Concentration
Whole blood in tubes that can be centrifuged for serum separation	For biochemical investigations like total protein, albumin, globulin, A/G ratio, total bilirubin, direct bilirubin, indirect bilirubin, BUN, creatinine, aspartate aminotransferase, alanine aminotransferase, total cholersterol, calcium, phosphorous, alkaline phosphatase etc.