Materials
10X labeling buffer
0.5M Tris-Cl, pH 7.5, 0.1M MgCl2, 10mM DTT, 0.5mg/ml BSA
dCTP, dGTP and dTTP (0.5mM in Tris-Cl pH 7.0)
a 32 P dATP – 3000 Ci/mmole
Klenow polymerase 10units/ml
Sephadex G-50
Silliconized glasswool
TE buffer
Last modified: Tuesday, 8 November 2011, 10:25 AM