Introductory Microbiology

Enzymes that associated with DNA

1) DNA polymerase: An enzyme that synthesize a new strand of DNA in 5’-3’ direction using the anti parallel strand as template.
2) Restriction endonuclease: An enzyme which recognizes and makes double strand DNA breaks at specific sequence of DNA.
3) RNA polymerase: An enzyme that synthesize RNA in 5’-3’ direction using antiparallel DNA strand as template.
4) DNA ligase: An enzyme that lygate or joint double strand DNA fragments.
5) DNA gyrase: This enzyme introduce super coiling of DNA in prokaryotes (also referred as topoisomerase-II).
6) Topoimerase-I: An enzyme which removes the super coiling of DNA (super coiling refers the highly twisted form of DNA).