Breeding of Fruit and Plantation Crops 2 + 1

Basic technique in plant cell culture

1. Callus and suspension culture
2. Haploid culture from pollen
3. Protoplast isolation and culture
4. Embryogenesis in cell culture
5. Selection of mutation from pathotoxin resistant cells and clones
6. Regeneration within heterogeneous materials
7. Regeneration of plants from somaclonal/protoclonal variation
8. Resistant plant through fusion of protoplast
9. Disease resistance through uptake of foreign genetic material

Genetic engineering or Recombinant DNA technology

• There is scope of genetic engineering in fruit crops for the development of transgenic varieties resistant to biotic/abiotic stresses. This technology involves the isolation of gene of desired character. Insertion of this isolated gene in a suitable vector (making it a recombinant vector).
  


• Insertion of the recombinant vector into a suitable host (organism/cell) known as transformation. Selection of the transformed host and multiplication followed by expression of the introduced gene into the host is the normal procedure adopted.