Principle

PRINCIPLE

Direct (or) Conjugated Bilirubin

  • It diazotizes with sulfanilic acid to form azobilirubin, a purple coloured complex in acid medium.

Indirect (or) Unconjugated Bilirubin

  • It is diazotized only in presence of methanol to give a purple coloured complex
  • Both direct and indirect reaction represents the total bilirubin concentration.
  • The difference of the total and direct gives the indirect bilirubin.
Last modified: Monday, 4 June 2012, 4:28 AM