Glucose

GLUCOSE

  • Normal urine does not contain glucose. Although glucose passes through the glomerulus, it should be reabsorbed by the proximal convoluted tubules.

Benedict’s test

  • Benedict’s qualitative reagent
  • Solution-I
    • Cupric sulphate crystals - 17.3 g
      Distilled water - 100 ml
  • Solution-II
    • Sodium carbonate - 100 g
      Sodium citrate - 173 g
      Distilled water - 700 ml
  • Cool the solution (II) to room temperature and pour into the solution (I), stirring constantly and make up the volume to 1000ml with distilled water.

Principle

  • Glucose is a reducing agent, which converts the cupric ions in the copper sulphate to cuprous ions, cuprous oxide.
  • Take 5 ml of the reagent in a test tube and heat it (the original blue colour of the reagent should not be changed)
  • Add 8 drops of urine to it and boil it carefully seeing that it does not spurt while boiling
  • Development of greenish, greenish-yellow, yellow or red color is positive for sugar  
BQR-Glu-Test

Interpretation - Glycosuria

    • Emotional stress
    • Diabetes mellitus
    • Hyperthyroidism
    • Acute and chronic pancreatic necrosis
    • Hyperpituitarism
    • Overactivity of adrenal cortex
    • Shock
    • Chronic liver disease
    • Enterotoxaemia in sheep
    • Brain tumors
    • Rabies
  • False positive reaction for glucose can occur if other reducing agents like antibiotics, lactose, ascorbic acid, salicylates, morphine, formaldehyde and uric acid are present.
Last modified: Monday, 4 June 2012, 4:41 AM