Dissection stage

DISSECTION STAGE

  • Position the specimen with its left side down, with the feet facing the examiner. Carefully examine the animal's exterior. Note the body openings for the presence of secretions/excretions, prolapse, and color of mucus membranes
  • Examine the hair coat, and note for the presence of ectoparasites, areas of alopecia, thickening of the skin, crust formations, tumour masses, and possible wounds
  • Penetrating wounds should be probed and the extent or depth noted. Palpate the continuity of bony structures and look for evidences of fractures and abnormal masses
  • Work with the right side of the specimen. The first incision is a straight line from the chin towards the ventral midline of the neck. Make an incision beginning from the chin and expose the mandibles and masseter muscles
  • Skin the neck and expose the underlying structures
  • Continue skinning backward to the flank of the right forelimb
  • Grasp and lift the right forelimb upward and cut all muscles between the subscapular area and the rib cage to free the limb
  • While doing this, locate, slice and examine the prescapular and axillary lymph glands
  • Examine the size and color and texture of the glands
  • After cutting all attachments of the forelimb, reflect the limb to the dorsum of the specimen
  • Hold the right hindlimb and cut the skin and underlying muscles of the hind flank
  • Expose the rim of the hip joint
  • With the aid of the tip of the knife, cut the round ligament to free the head of the femur and consequently the hindlimb
  • While doing this, take note of the articular surfaces of the acetabulum and the femur
  • Note also the amount, quality and quantity of the synovial fluid, and the appearance of the joint structures.
  • Reflect the freed hindlimb to the dorsum of the specimen
  • Continue skinning the ventral midline of the specimen from the incision made at the region of the rear flank and backward to the hind flank area
  • Reflect the skin at the dorsum of the specimen
  • While skinning the specimen, take note of the quality of the carcass in terms of the amount and appearance of the flesh
  • Note for any discoloration, bruises and prior bleeding points.
  • The next step is to open the buccal cavity
  • To do this, cut deeply the submandibular muscles and underlying structures close to the inner rims of the mandible at both sides
  • With the aid of an ordinary pruning shear or costotome, split the mandibles at its symphysis
  • Alternatively, a hacksaw may be used to do this
  • Grasp the tongue and pull it backward
  • Cut all muscular attachments to free the tongue
  • Severe the hyoid bones at the articulation of the great and the small corns
  • Free the tongue by cutting all structures behind the tonsillar tissues
  • Examine the palate, pharyngeal mucosa, and tonsillar tissues
  • Drag the tongue backward and dissect the trachea and oesophagus cutting all attachments up to the thoracic inlet
  • Leave the freed tongue, oesophagus and trachea still attached at the thoracic inlet
  • The abdominal cavity is then opened
  • Palpate the free edges of the last rib and make a shallow incision sufficient to cut the abdominal muscles and peritoneum at this region while not cutting deeper structures
  • Lift the opening and continue cutting the abdominal wall from the dorsum and into the area of the xiphoid cartilage of the sternum
  • Continue cutting the abdominal wall at its dorsal and caudal boundaries down to the inguinal region
  • While this is done, note for the presence of ascitic fluid
  • Save as much fluid as possible for measurement of volume
  • Be careful not to cut the intestinal segments while doing this maneuver
  • After exposing the abdominal organs, leave the omentum that covers the intestinal loops for a while to avoid drying of the exposed segments.
  • Position the knife at the angle formed by sternal part of the diaphragm and the xiphoid cartilage of the sternum
  • Cut the sternal part of the diaphragm and note the presence or absence of negative pressure within the thoracic cavity
  • The presence of a negative pressure is suggested by the backward displacement of the diaphragm
  • Continue cutting the costal part of the diaphragm close to the inner rims of the ribs
  • The right side of the rib cage is then severed from its attachment to the sternum
  • Cut the costo-chondral articulation from the last articulation and towards the first rib
  • Be careful not to severe the tongue, trachea and oesophagus lying freely at this region
  • Detach the wall of the rib cage by cutting the neck of the ribs and associated intercostal muscles to expose the thoracic organs
  • The pelvic cavity is then opened
  • This is done by sawing the ilium close to the rim of the acetabulum at both sides
  • Then, saw the ischium from behind at both sides
  • Remove the sawed portion of the pelvic bone by cutting all underlying attachments to expose the pelvic cavity and its contents

At this point, the buccal, thoracic, abdominal and pelvic cavities and their contents are exposed for detailed examination

Last modified: Friday, 23 March 2012, 10:04 AM