Special Veterinary Pathology

COLLECTION OF BLOOD AND BODY FLUIDS

• Blood samples should be collected prior to euthanasia of moribund animals
• In some cases, blood samples may still be obtained in animals that have been dead from three to four hours
• This is done by aspirating the blood contained in the heart prior to detachment and dissection of the chambers
• The addition of anticoagulants (e.g.,ethylenediaminetetraacetic acid or EDTA) should be considered if plasma is to be extracted.
• If sera are required, it is best to collect blood in a glass receptacle to promote clotting.
• Alternatively, glass beads may be added.
• The general rule in collecting body fluid is to obtain samples free from contaminants.
• Body fluids should be collected as the examination progresses if it is anticipated that such examination is required.
• Ascitic fluid should be qualified as to amount, colour and turbidity.
• Aspirating directly from the urinary bladder may collect urine.
• Cerebrospinal fluid should be collected prior to opening the brain
• This should be done by aspirating the fluid through the aid of syringe and needle at the cisterna magna.