Systematic Veterinary Bacteriology and Mycology

CULTIVATION

Cultivation of the organism

• The Aspergillus grows very well in ordinary Sabouraud's Dextrose Agar with chloramphenicol.
• Cycloheximide should never been incorporated in the media.

Characters	A.fumigatus	A.niger	A.flavus
Macroscopic morphology of the colony	Velvetty or powdery, at first white then turning to dark bluish green. Reverse of the colony will be white to tan.	Wooly. At first white to yellow and then turning to dark brown to black. Reverse: white to yellow	Velvetty, yellow to green or brown Reverse: Red brown
Morphology of conidiospore and sterigmata	Single, usually one on upper half of the vesicle, parellel to axis of stalk.	Double, cover entire vesicle, form radiate head	Single and double, cover entire vesicle, point out in all directions