Systematic Veterinary Bacteriology and Mycology

DIAGNOSIS

• Diagnosis required continuous effort because it is one of the common contaminant of laboratory and it can be cultured routinely from the skin and URT of healthy animals.
• For confirmatory diagnosis, consider the following points:
  • Repeated isolation
  • Absence of any other pathogen
  • Recovery from unexposed tissue and demonstration of hyphae.

Method of diagnosis

• Direct microscopic examination
  • Wet mount prepartion of sputum, nasal discharge, milk, uterine discharge, fetal stomach contents.
• Diagnosis is confirmed by isolation of pathogens from the stomach contents of the aborted foetuses and placenta.
  • Demonstration of fungal hyphae in the foetal tissue (By using 10% KOH or LPCB).
• Demonstration of pattern of condiospore and sterigmata by slide culture method.
• Animal pathogenicity test
• Allergic test.