CAMP test
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This test is used to differentiate S. agalactiae from other streptococci.
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The CAMP phenomenon is characterized by a zone of incomplete haemolysis produced by Staphylococcus, later becoming completely lysed by streptococcal haemolysin to produce an arrowhead appearance.
The CAMP test is performed as follows.
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Streak (a zone of partially haemolytic) Staphylococcus aureus as a straight line on blood agar containing esculin.
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Streak the suspected streptococci at a right angle to the Staphylococcus aureus line.
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Incubate at 37°C for 18-24 hours until growth is evident.
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Examine for arrowhead haemolysis.
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Esculin may be incorporated into the blood agar to test for esculin hydrolysis at the same time.
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A positive reaction is seen as brown discoloration of the medium around the colonies.
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Organism
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CAMP
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Esculin
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S. agalactiae
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+
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-
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S. dysgalactiae
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-
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-
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S. uberis
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-
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+
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Enterococcus faecalis
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-
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+
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Last modified: Monday, 4 June 2012, 6:20 AM