Animal Biotechnology

TRANSLATION IN EUKARYOTES

Initiation in Eukaryotes

• Most of the differences in the mechanism of protein synthesis between prokaryotes and eukaryotes occur in the initiation stage.Eukaryotes have atleast 9 eIF.
• Eukaryotic initiator tRNA does not become formylated as in prokaryotes.
• Since there is no Shine-Dalgarno sequence in Eukaryotic mRNA, the 40S subunit containing the initiator tRNA attaches to the 5' end of the mRNA and scans along the mRNA until it finds an appropriate AUG. (Kozak’s scanning mechanism)

Elongation

• The factors eEF1 a ,eEF1 b g and eEF2 are involved in elongation.

Termination

• Eukaryotes have only one release factor eRF which recognise all the stop codons.