Animal Biotechnology

STEPS IN RECOMBINANT DNA TECHNIQUE / GENE CLONING

• The entire procedure of gene cloning or recombinant DNA technology may be classified into the following five steps
  • Identification and isolation of the desired gene or DNA fragment to be cloned.
  • Insertion of the isolated gene in a suitable vector.
  • Introduction of this vector into a suitable organism/cell called host (transformation).
  • Selection of the transformed host cells.
  • Multiplication /expression/integration followed by expression of the introduced gene in the host.