Animal Biotechnology

GENERAL STEPS IN CLONING

• Prepare the vector and the DNA to be cloned by digestion with restriction enzymes to generate complementary ends.
• Ligate (join) the foreign DNA into the vector with the enzyme DNA ligase.
• Introduce the DNA into bacterial cells or yeast cells by transformation.
• Select cells containing foreign DNA by screening for selectable  markers (commonly drug resistance).

Steps in cloning