RFLP

RESTRICTION FRAGMENT LENGTH POLYMORPHISM (RFLP)
  • Restriction fragment length polymorphism (RFLP) analyzes the length of the strands of the DNA molecules with repeating base pair patterns.
  • While about 5% of DNA contains the genes, the other 95% is considered as non-coding genes (junk DNA) but contains identifiable repetitive sequences of base pairs, which are called Variable Number Tandem Repeats (VNTR).
  • To extract a DNA fingerprint, a Southern blot is performed and the DNA is analyzed via a radioactive probe.
  • The restriction fragment length polymorphism analysis is used to detect the repeated sequences by determining a specific pattern to the VNTR, which becomes the person's DNA fingerprint.
  • The drawback with this system is that it requires a considerable amount of DNA in order to be used.
Last modified: Tuesday, 13 September 2011, 11:09 AM