Test for Glucose

TEST FOR GLUCOSE

Benedict’s test

  • This test is for the detection of reducing sugars (Glucose).
  • Take 2.5 ml of Benedict’s qualitative reagent in a test tube
  • Add 4 drops of urine, boil for 2 minutes and then cool.
  • If glucose is present, gradually a precipitate with green, yellow, orange or brick red colors may be formed depending on the concentration of glucose present.

Interpretation - Glycosuria

    • Emotional stress
    • Diabetes mellitus
    • Hyperthyroidism
    • Acute and chronic pancreatic necrosis
    • Hyperpituitarism
    • Overactivity of adrenal cortex
    • Shock
    • Chronic liver disease
    • Enterotoxaemia in sheep
    • Brain tumors
    • Rabies
  • False positive reaction for glucose can occur if other reducing agents like antibiotics, lactose, ascorbic acid, salicylates, morphine, formaldehyde and uric acid are present.
Last modified: Thursday, 1 December 2011, 7:08 AM