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Estimation of serum chloride
Chloride ions react with a solution of mercuric thiocyanate and ferric nitrate to form undissociated mercuric chloride and a red brown ferric thiocyanate complex, which is quantitatively proportional to the chloride ion concentration. 2 Cl- + Hg (SCN)2 → HgCl2 + 2 (SCN)- 3 (SCN)- + Fe3+ → Fe (SCN)3 Reagents:
Pipette into two clean, dry test tubes labelled blank (B), standard (S), and test (T) as follows:
Mix well, wait for 1 minute at room temperature and read the absorbance of standard (S) and test (T) against blank at 510nm or with a green filter in a photoelectric colorimeter within 60 minutes. Calculations : Chloride (mmol/L) = O.D (T) ----------- x 100 O.D (S) Result: The Chloride content of given serum = mmol / l or meq / l Clinical Significance:
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