Veterinary Clinical Biochemistry and Laboratory Diagnosis-II

MATERIALS FOR BACTERIAL DISEASES

Si.No	Diseases	Clinical Materials
1	Actinobacillosis	Smears of pus from deeper portion of the lesions-heat fixed Swab of pus from abscess on ice A piece of affected tongue on ice.
2	Actinomycosis	Smears of pus from deeper portion of the lesions-heat fixed Pus in sterile tubes Swab of pus from abscess on ice
3	Anthrax	Peripheral blood smear, swab of blood, exudates from natural orifices, Putrified case - ear or muzzle pieces in boric acid
4	Black quarter	Impression smear from affected muscle, muscle piece in sterile container on ice, smears of exudate from thigh
5	Bovine genital  camphylobacteriosis	Male: Prepucial mucus, smegma, semen, prepucial washing Female: Vaginal mucus, aborted fetus, fetal stomach contents, Lung, Liver and Placenta
6	Brucellosis	Live animal: Serum, blood, Milk, Joint fluid, lymph node. Abortion: Placenta, Cotyledons & vaginal discharge Foetus: Smear from abdominal fluid, fetal stomach contents, lung, liver on ice.
7	Hemorrhagic   septicemia	Auricular vein smear, Oedema fluid smear, Heart blood smear fixed in methanol. Swab from Heart blood, pleura, lung in transport medium Piece of lung and spleen in sterile container on ice and long bone in case of putrifaction in charcoal
8	Johne’s Disease	Live animal: Rectal pinch smear, Rectal washing,dung samples in sterile vial. Dead Animal: Intestinal contents, Lymph nodes on ice, Mucosal smears from thickened areas of intestine or ileocecal valve.
9	Leptospirosis	Blood, serum, milk and urine Internal organs: kidney, Liver, Lung, Brain or adrenal gland on ice.
10	Listeriosis	Swab from nasal cavity and heart blood Foetus: Swab fromheart blood,brain on ice
11	Mastitis	Milk from affected quarter on ice
12	Tuberculosis	Smears from the sputum, lymph node and lesions fixed by heat, Swab from lesions in transport medium, TB nodule from any visceral organ and pieces of organs along with lymphnode on ice.
13	Enterotoxaemia	Intestinal contents(10ml) preserved with 3 to 4 drop of chloroform , loop (12") of intestine tied at both ends on ice,Impression smear from kidney, smear from intestinal mucosa, liver, lung,kidney, brain on ice, Urine (5ml) preserved with thymol
14	Colibacillosis	Cloacal swabs, Intestinal swab and Heart blood swab in transport medium, intestinal content, mesentric lymphnode, liver, spleen, kidney on ice. Poultry: Swab from liver, lung,airsac, oviduct, yolk sac
15	Fowl cholera	Smear heart blood, exudate, liver and spleen swab from heart blood ,liver, lung, spleen, cloaca in transport medium, Visceral organs, bone marrow on ice.
16	Fowl typhoid and   Pullorum	Live bird: Whole blood, serum, swab from egg yolk, meconium, Cloacal swab, intestinal swab, visceral organs, piece of intestine tied at both .
17	Chronic respiratory   disesase	Swab from oropharynx, trachea,cloaca Serum from conmvalescent birds Dead bird: Swab from nasal cavity, infraorbital sinus, trachea, lungs, air sac, joint cavity, yolk or embryo.