Laboratory Diagnosis

LABORATORY DIAGNOSIS
  • Specimens to be collected: specimens should preferably be collected from animals with a high fever and oral lesions.  The following samples should be collected from live animals
  • Live animals
    • Blood in EDTA or heparin
    • Blood for serum
    • Swabs containing lacrimal fluid
    • Tears
    • Necrotic tissue from the oral cavity
    • Aspiration biopsies of superficial lymph nodes
  • Dead animals: Sections of
    • Spleen
    • Lymph nodes
    • Tonsil
  • Isolation and identification of virus in calf kidney cells and vero cells using specimens specified above.
  • Detection of virus specific antigen by
    • Agar gel immunodiffusion test
    • Counter immunoelectrophoresis
    • Passive haemagglutination
    • Immunofluorescense and immunoperoxidase techniques
    • Antigen capture ELISA
    • RT-PCR
    • DNA probes for N gene
Last modified: Tuesday, 28 September 2010, 11:12 AM