Systematic Veterinary Virology

Laboratory Diagnosis

LABORATORY DIAGNSOSIS

• Isolation and identification
• Clinical materials: Specimens for diagnosis are heparinized blood, serum (paired [acute and convalescent] sera if animal survives), and half the brain and piece of pancreas unfixed and a completed set of tissues in 10 percent formalin.
• Isolation systems: Newborn mice, embryonted eggs and cell lines like Vero, BHK21, RK13.
• Antigen identification:
• Complement fixation test
• RT-PCR
• Antigen capture ELISA
• Immunofluorescence
• Identification of viral nucleic acid in mosquitoes by RT-PCR.