Systematic Veterinary Virology

SEROLOGICAL TESTS

• Serological confirmation of EEE, WEE and VEE virus infection is confirmed by a four-fold or greater increase or decrease in antibody titre in paired serum samples collected 10–14 days apart. The common serological tests performed are as below:
• Complement fixation test
• Haemagglutination inhibition test using male geese RBCs
• ELISA
• Plaque reduction neutralization test (PRN test) - The PRN test is very specific and can be used to differentiate between EEE and WEE virus infections. The PRN test is performed in duck embryo fibroblast, Vero, or BHK-21 cell cultures.