Cultivation

CULTIVATION

  • ECE
    • The virus are cultivated in ECE by chorioallantoic membrane route. Appearance of focal white pock lesions or generalised thickening of the CAMs after 5-7 days is the characteristic lesion. Histopathological examination of the CAM lesions will reveal eosinopilic intracytoplasmic inclusion bodies following staining with H& E
  • Cell culture
    • Primary chicken embryo fibroblasts, chicken embryo kidney cells, chicken embryo dermis cells, or the permanent quail cell line QT-35, can also be used to propagate fowlpox virus. Production of pock is the characteristic CPE
Last modified: Friday, 1 October 2010, 5:57 AM