Systematic Veterinary Virology

LABORATORY DIAGNOSIS

• Isolation and identification
• Clinical materials: Heparinized blood, Clotted blood or serum, Submandibular lymph node, Inguinal lymph node, Tonsil, Spleen, Gastrohepatic lymph node, Lung, Liver, kidney and bone marrow.
• Isolation system: Primary pig cell cultures like pig monocytes and bone marrow cells or susceptible pigs
• Identification: Presence of virus in cell cultures can be demonstrated by adding red blood cells to the culture. These are attracted to the surface of infected cells, to which they cling and form “rosettes”, a phenomenon known as haemadsorption. The viruses are also identified by immunofluorescense or by PCR.
• Virus detection in tissue samples
• Immunofluorescense
• Immunoperoxidase staining
• PCR