Sandwich ELISA

SANDWICH ELISA

   A less-common variant of this technique, called "sandwich" ELISA, is used  to detect sample antigen. The steps are as follows:

  • Plate is coated with a capture antibody
  • Sample is added, and any antigen present binds to capture antibody
  • Detecting antibody (raised in a different species other than the capture antibody) is added, and binds to antigen
  • Enzyme-linked secondary antibody is added, and binds to detecting antibody
  • Substrate is added, and is converted by enzyme to detectable form.
Last modified: Saturday, 3 December 2011, 9:30 AM