Procedure
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Pour the agarose solution in to the clean Petri plate and allow to solidify for 15-20 minutes.
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After solidification punch the wells using gel puncher (The distances between the wells are important. Try to follow the template as accurately as possible).
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Fill the center well with 40 μl of positive serum and side wells with 30 μl of test antigen in two wells and positive and negative antigen control in each well.
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Cover the plate and place carefully (do not invert) inside the moist chamber (box containing wet cotton) and incubate it at 37°C for overnight or at room temperature for 24 to 48 hours.
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The precipitin lines will be visible in 24-48 hours.
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Last modified: Tuesday, 29 March 2011, 11:42 AM