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Diseases
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Materials to be collected
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Bovines
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Rinderpest
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Defibrinated or citrated blood at the height of temperature on ice, lymph glands and spleen on ice. Intestinal lesions, lymph nodes in 10% formal saline (Materials should never be collected in 50% buffered glycerine)
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Foot and mouth disease
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Vesicular fluid in transport medium, vesicular epithelium from fresh lesions in foot and mouth viz. tongue, gum, dental pad lesions in 50% buffered glycerine
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Mucosal disease and Bovine viral diarrhoea
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Defibrinated and citrated blood at the peak of temperature and lesions from mouth, gastrointestinal tract, spleen, liver and kidney in 50% buffered saline
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Infectious bovine rhinotracheitis/infectious pustular vulvovaginits
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Nasal, conjunctival and genital, swabs and foetal tissues in transport medium (Hank’s medium), paired serum samples. Affected trachea, turbinate bones, foetal tissues etc. in 10% formal saline and in transport medium
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Cow pox
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Skin scrapings and vesicular fluid in transport medium (Hank’s medium)
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Bovine malignant catarrhal fever
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Citrated or defibrinated blood, lymph nodes of head, neck, pieces of liver, spleen on ice. Pieces of brain, liver, spleen, kidney, adrenals, nasal mucosa in 10% formalin
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Ephemeral fever
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Heparinized, citrated or defrinated blood on ice, paired sera samples
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Influenza and para-influenza
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Nasal swabs in Hank’s medium and paired serum samples
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Rabies
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As given in canines
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Ovine and Caprine
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Pox
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As in bovines
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Bluetongue
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Nasal swabs, tongue epithelium in Hank’s medium, paired sera samples, citrated blood etc.
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Louping ill (encephalomyelitis)
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Citrated blood at height of temperature on ice, paired sera samples, brain in 50% buffered glycerine
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Visna and maedi
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Citrated blood, cerebrospinal fluid and saliva in Hank’s medium, brain, lungs and spleen in 50 % glycerine saline
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Equine
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African horse sickness
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Citrated blood at height of temperature on ice, paired sera samples, spleen, lymph nodes, brain on ice
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Equine Herpes virus –I
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Nasal, vaginal, conjunctical swabs, aborted material from foetus in Hank’s medium, paired sera samples
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Influenza
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As in Bovines
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Equine encephalomyelitis
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Citrated blood, brain on ice, paired sera samples
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Equine infectious anemia
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Serum, whole blood on ice
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Rabies
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As in canines
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Canines
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Rabies
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Half of the brain in 50% glycerine saline on ice packed carefully sent through courier. Brain fixed in 10% formalin, smear of brain tissue fixed in methyl alcohol
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Infectious canine Hepatitis
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Lung, liver, kidney and urinary bladder in transport media and 10 % formalin. Paired sera samples
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Canine distemper
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Conjunctival swabs and scrapings from gums in Hank’s medium, gum smears on slides, pieces of lung, liver, bladder, trachea, stomach in Hank’s medium and in 10% formalin
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Canine parvo virus
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Pieces of liver, spleen, intestines, stomach, mesenteric lymph nodes in 50% glycerine saline
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Porcine
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Swine fever
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Citrated blood, pancreas, half brain, tonsils, spleen, lymph nodes on ice and 50% glycerine saline and 10% formalin
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Viral pneumonia
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Pleural exudates in Hank’s medium, lungs, mediastinal lymph nodes on ice and 10% formalin
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Vesicular exanthema and vesicular stomatitis
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Vesicular fluids, tissues from snout, lips, foot pad lesions in Hank’s medium on ice
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Rabies, Aujesky’s disease and Teschen disease
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Duplicate specimen of brain, spinal cord, spleen, and p[ortion of faeces on ice and 10 % formalin saline. Blood serum
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Swine influenza
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Nasal swabs and lung tracheal tissues on ice
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Poultry
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Infectious Bronchitis/infectious laryngotracheitis
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Whole bird or duplicate portion of trachea, lung and kidney on ice and 10% formalin
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Avian encephalitis
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Whole bird, bursa, spleen, and thymus on ice
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Newcastle disease
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Whole bird or brain, spleen, liver, caecal tonsils, intestines in 50% glycerine, serum samples from acute and convalescent cases
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Fowl pox
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Comb, skin, eyelids with scabs in 10 % formal saline. Scabs and pustules in 50 % glycerine saline
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Marek’s disease
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Affected bird, peripheral nerves, brain, ovary, lung, liver, kidney, spleen, heart in 10 % formal saline. Skin and feather in 10 % formal saline. Skin and feather tips from crural region as such
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Gumboro disease
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Whole bird, bursa, spleen and thymus on ice
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Participants