Veterinary Clinical Medicine-I (General and Systemic)

TRYPSIN - LIKE IMMUNOREACTIVITY

• Measurement of serum trypsin-like immunoreactivity (cTLI) by radioimmunoassay.
• The cTLI is a species and pancreas specific test and determines trypsin and trypsinogen that have entered the blood from pancreas.
• The values of TLI test are characteristic and help in differentiating EPI of small intestinal diseases.
• Low-fasting serum cTLI concentrations (< 2.5 μg/L) are typical of EPI.
• Reference range of TLI in normal dogs is 5.0 to 35 μg/L. cTLI values between 2.5-5 μg/L are indicative of early phase of EPI, before severe destruction of exocrine pancreas.
• A new fecal test for diagnosis of EPI is based on the determination of fecal elastase activity using the ELISA method. It is also a species and pancreas--specific test. A single fecal sample is sufficient and values less than 10 μg/g are suggestive of EPI.
• The fasting serum feline TLI test (fTLI) : Values of less than 8 μg/L are suggestive of EPI. fTLI values between 8-17 μg/L are indicative of early phase of EPI.