Diagnostic Laboratory Section

EXERCISE

1. What do you mean by acid fastness?
2. Why tubercle bacilli are acid fast ?
3. Why heating of carbol fuchsin is necessary in Ziehl Neelsen staining?
4. What are the other methods of acid fast staining ?.
5. Can we decolourize the smear with acids other than sulphuric acid ?.
6. What is the pupose of fixing bacterial smear?
7. Name some basic dyes used for bacterial staining ?
8. Name the acid dye used in bacterial staining?
9. Name various decolorising agents?
10. What is mordant?

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