Breeding of Vegetable, Spice and Tuber crops (2+1)

Embryo culture

Removal of developing young embryos from seeds and their cultivation in vitro.Exchange of genes between cultivated and wild species and transfer of resistant gene(s) from wild species to cultivated varieties is hampered due to certain barriers in sexual hybridization.Therefore, the in vitro method has been successfully used to overcome such barriers in distant hybridization. In this case, hybrid embryo are rescued prior to their abortion and cultured directly into artificial media.The embryo culture technique has been practically utilized for proper development of fertilized embryos in following cases :Solanum lycopersicum syn (Lycopersicon esculentum) x L.peruvianum

• Capsicum pendulum x C.annuum
• Phaseolus vulgaris x P.acutifolius
• Cucurbita pepo x C.ecuadorensis
• Cucurbita metuliferus x C.zeyheri
• Abelmoschus esculentus x A. moschatus
• Abelmoschus esculentus x A.ficulneus