Veterinary Andrology and Reproductive Techniques

PYRUVATE UTILISATION TEST

• Found by Melrose during 1952.
• The quality of semen samples could be graded according to the oxygen consumption after the addition of pyruvate and pyruvate plus 2:4 dinitrophenol. Flouride was added to the sample to reduce the exogenous metaboloism to a low level.
• When added with pyruvate the oxygen consumption in high fertile bull semen and low fertile bull semen is increased but on addition of 2:4 dinitrophenol the oxygen uptake is increasing two fold in high fertile bulls but not in low fertile bulls.
• The oxygen uptake was measured with the help of monometric equipment.