Veterinary Andrology and Reproductive Techniques

POST-THAW MOTILITY

• Post thaw motility is examined 24 hours after freezing the semen.
• Thaw the frozen semen straw at a water bath of 37⁰C for 30 seconds.
• Wipe the straw and cut the ends of the straw and collect the semen in a small test tube.
• Keep a small drop on a clean, grease free glass slide.
• Cover it with cover slip and examine under the phase contrast microscope.
• Motility based on progressive motile sperms is evaluated on the nearest 5 percentage.
• If motility differs by 10 per cent or more between two straws of the same batch a third straw is examined.

The minimum post thaw motility of 50 percentage is acceptable.

• In routine practice only post thaw motility is performed and it is the most reliable test when carried out by an experienced person.

Click the links below to view video:

• PTM -10% (Buffalo semen)
• PTM -50-60% (Buffalo semen)
• PTM -60-70% (Buffalo semen)
• PTM-Jerky and circular (Buffalo semen)
• PTM -50-60% (Bull semen)
• PTM -60-70% (Bull semen)

Note: The following three tests are done only as added tests when it is suspected that semen may be of lowered fertility but meets minimum standards for fertility.

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