Veterinary Andrology and Reproductive Techniques

CALCULATION OF DILUTION RATE

The dilution rate is calculated according to the number of sperms to be packed in a straw. In both French mini and medium straws 20 million sperms are packed.

For French mini straw

• The French mini straw is having 20 million sperms in 0.25 ml.
• Let us consider the

• The total diluted volume is 62.5 ml which includes the semen volume also. So to the 5 ml semen 57.5 ml diluents is added to make the final volume of 62.5 ml.

TOP

For French medium straw

• The French medium straw is having 20 million sperms in 0.5 ml.
• Let us consider the

• The total diluted volume is 125 ml which includes the semen volume also. So to the 5 ml semen 120 ml diluents is added to make the final volume of 125 ml.

TOP

Dilution of semen

• Immediately after semen collection the volume, concentration and initial motility are estimated.
• A minimum of 2.5 ml volume, 500 million concentration and 70% initial motility is required to process a semen sample.
• The semen is diluted initially with 1:1 dilution for assessing the initial motility.
• Then the dilution rate is calculated as per the above method (depends upon the packing volume) and the final dilution is made in a sterile beaker.
• While diluting the semen the diluents should not be poured directly over the semen.
• It should be poured in the side walls of the beaker without air bubble formation.

TOP