Veterinary Andrology and Reproductive Techniques

THAWING FRENCH SEMEN STRAW

• Lift the neck plug of the LN2 container
• Precool the forceps in the liquid nitrogen vapour for 5-10 seconds
• Lift the canister from the neck and move it to its opposite edge. Now the body of the canister will come to centre
• Lift the canister to the level of neck
• Identify and lift the straw by forceps
• The whole operation of lifting of canister and removal of straw should be done as fast as possible ie. with in 5-10 seconds. Otherwise immerse the canister into the liquid nitrogen.
• Immediately after removing the semen straw give a jerk to remove the liquid nitrogen on the straw and also to avoid popping of the seal.
• If the straw is having cracks or broken or defective seals the straw should be discarded.

Put the semen straw in a water bath of 37 degree celsius for 30 seconds .

• While thawing the semen straw should be completely submerged in water.
• The thawing water should be fresh and clean. The container used to hold the thawing water should also be clean.
• Thaw the semen straw either in horizontal or vertical manner
• After the thawing, remove the straw and wipe with cotton to remove all the water on the surface of the straw.

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