Veterinary Andrology and Reproductive Techniques

DOGS

• AI in the canine generally is based on the use of fresh, unextended semen.
• Generally, the entire sperm rich fraction is deposited into the anterior vagina of the estrus female.
• A plastic pipette is passed dorso-cranially into the vagina of the bitch to the external cervical os.
• The semen is slowly injected, followed by elevation of the bitch’s hindquarters for 5 minutes. Elevation helps prevent the premature loss of semen from the vaginal tract.

Intracervical or intrauterine insemination via the vagina is nearly impossible.

• Vaginal stimulation with a gloved finger may aid in the transport of semen into the uterus via genital contractions and altering vaginal pressures.
• The use of transvaginal insemination with frozen semen has produced extremely erratic results in conception rate and litter size.

• The most consistent results are obtained through surgical intrauterine insemination of frozen semen containing 100×10⁶ spermatozoa.

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