Veterinary Andrology and Reproductive Techniques

BACTERIOLOGICAL MEDIA

Use standard plate count agar to assess the bacterial load of semen (both fresh and frozen).

• Standard plate count agar is used for bacteriological examination of the fresh, frozen semen and also for the consumeables used in semen bank.
• The media is autoclaved at 15 psi pressure for 15 minutes.
• The entire procedure should be done under laminar air flow unit to avoid false positive results.