Veterinary Andrology and Reproductive Techniques

METHODS OF ASSESSING SPERM MORPHOLOGY

Sperm morphology is assessed by

• Eosin-Nigrosin stain
• Rose Bengal stain
• Indian ink stain
• Wright’s stain
• Casaretts’s stain
• Buffered formal saline method (wet smear method)

• An eosin-nigrosin stain is commonly used as a morphology stain.
• The eosin-nigrosin sperm smears prepared for live and dead count is satisfactory for assessing abnormal spermatozoa.