Veterinary Andrology and Reproductive Techniques

D2 DILUTOR

Found by Singh and Tomar (1959).

• Singh and Tomar (1959) modified the above dilutor and named it as D₂ dilutor. The composition is as follows.

S.No.	Components	Solution	Quantity
1	Sodium bicarbonate	1.3 % solution	10 parts
2	Glucose anhydrous	5 % solution	40 parts
3	Fructose	5 % solution	25 parts
4	Egg yolk		25 parts

• Tomar and Desai (1961) claimed it to be efficient medium for buffalo semen.
• Tomar et al. (1968) used the above D₂ medium in routine practice for buffalo and Zebu semen.
• Buffalo semen was maintained fit for use for 4 days while Zebu semen was preserved for 5-6 days.
• Fertility ranged around 45% in Zebu and 40% in buffaloes.