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Sample Preparation
Aseptically transfer 1 ml of the culture from the broth medium into 9 ml of diluent (Normal Saline). This gives a 10-1 dilution. Mix the diluent and the culture well using a vortex mixer or rolling in between your palms. Again transfer 1 ml of the diluted culture from 10-1 dilution to another 9 ml diluent tube and mix well (10-2). Repeat this procedure till the required dilutions are made (up to 10-6 ). |
Last modified: Thursday, 10 November 2011, 4:17 AM