FPT 111: Method

Method

Add 0.1g of soil/sediment sample 0.1ml of pond water to nitrite formation medium (adjust pH to neutral of slightly alkaline) and incubate at 25ºC for seven days.

Test the culture for presence of nitrites by adding three drops of Trommsdorf solution with one drop of sulphuric acid to a drop of culture.

Positive test is indicated by the appearance of intense blue-black colour.

Test for ammonia with Nessler’s reagent.

Negative result for ammonia indicates complete oxidation of ammonia to nitrites. Isolate and develop pure cultures.

Make a gram-stain preparation to observe morphology.

Last modified: Thursday, 10 November 2011, 5:12 AM

Home
- Site pages
  - Tags
  - Calendar
  - Site news
- Current course
  - FPT 111
    - Participants
    - General
    - Topic 1
    - Topic 2
    - Topic 3
    - Topic 4
    - Topic 5
    - Topic 6
    - Topic 7
    - Topic 8
    - Topic 9
    - Topic 10
    - Topic 11
    - Topic 12
    - Topic 13
    - Topic 14
    - Topic 15
    - Topic 16
    - Topic 17
    - Topic 18
    - Topic 19
    - Topic 20
    - Topic 21
    - Topic 22
    - Topic 23
    - Topic 24
    - Topic 25
    - Topic 26
    - Topic 27
    - Topic 28
    - Topic 29
    - Topic 30
    - Topic 31
    - Topic 32
      - Aim
      - Requirements
      - Method
      - Observation
    - Topic 33
    - Topic 34
    - Topic 35
    - Topic 36
    - Topic 37
    - Topic 38
    - Topic 39
- Courses

Fundamentals of Microbiology (2+2)

Method

Navigation