Biotechnology and Bioinformatics (1+1)

Note

1. For extracting DNA from fish blood insert 2 ml syringe containing anticoagulant near the caudal region below the lateral line and with draw 0.5 ml blood from the caudal vein and transfer to eppendorf tube. After adding lysis buffer keep in a water bath at 60 ° C for 1-2 hr. Proceed with phenol–chloroform extraction.

2. SDS- Don’t keep it in refrigerator, it will get precipitate. If stored in refrigerator before use warm it. Cap it airtight and store on the work bench.