Biotechnology and Bioinformatics (1+1)

1. Solutions required

50X TAE (generally diluted to 1X for use) :

242 g Tris; 700 ml ddH2O; 57.1ml glacial acetic acid; 100 ml 0.5 M EDTA pH 8.0. Adjust volume to 1L using dd H2O. Autoclave and store at room temperature.

1X TAE agarose gel (1%) :

2 g agarose; 200 ml 1 X TAE; heat in microwave oven until agarose dissolved (do not boil). Store in 65°C oven.

Load dye : 50 mM EDTA; 30% Glycerol; 0.25% bromophenol blue; 0.25% xylene cyanol

Marker : 50 mM EDTA; 20 μl load dye; 175 μl 1 X TAE; 5 μl DNA marker (HindIII digest of λ phage DNA for example)

Ethidium bromide : HAZARD!!! Wear double gloves when handling.