Biotechnology and Bioinformatics (1+1)

Trouble shooting

1. Poor polymerization: Polymerization is inhibited by contamination with impurities in the water or reagent and contaminants from the glass, comb, spacers or tubing. Ammonium per sulphate is highly unstable. So store the crystals in a desiccator. Every time prepare freshly or store the solution at 1- 20°C in small aliquots

2. Protein overload: This will result in distorted bands

3. Wavy bands: This is because of excessive heating of the sample. To avoid heating run the gel at low current and low temperature. Inadequate polymerization will also cause this.