Biotechnology and Bioinformatics (1+1)

Microwell assays

Assays for antigens or antibodies are routinely performed in plastic (PVC or polystyrene) microwell plates. The basis for these assays is the fact that proteins adsorb to the surfaces of many plastics and will remain adsorbed throughout subsequent incubation steps. A micro well assay in which an antiserum is bound to the well via protein A or protein G and the antibody of interest is detected using an enzyme conjugate.

Assays for antigens or antibodies are routinely performed in plastic (PVC or polystyrene) microwell plates. The basis for these assays is the fact that proteins adsorb to the surfaces of many plastics and will remain adsorbed throughout subsequent incubation steps. A micro well assay in which an antiserum is bound to the well via protein A or protein G and the antibody of interest is detected using an enzyme conjugate.