Biotechnology and Bioinformatics (1+1)

Procedure

1 . SDS-PAGE

1. A separating gel (10%) was cast in the gel assembly system and a small column of stacking gel was poured over it and an appropriate comb was placed and kept for polymerization. The gel was pre run for 10 min at . 100 V

2. Samples [Extracellular products of hemolysin isolates from Aeromonas hydrophila] are prepared by boiling at 100 ' C with an equal Volume of sample buffer for 3-5 min. Electrophoresis is done at 80-100 V till the dye front reached the bottom of the gel .

3. After electrophoresis, the gel was kept in the staining solution (0 . 001 % ­Coomassie brilliant blue R-250) for 2h or overnight and the excess stain was removed by destaining till the bands were clear .