Materials

Materials

Depurination solution: 0.25M HCL

Denaturation solution: 1.5M NaCl, 0.5 M NaOH (store at Room temperatue)

Neutralization solution: 1.5M NaCl, 1M Tris Cl, pH 7.5 (store at Room temperatue )

20X and 2X SSC

Nylon or nitrocellulose membrane

Last modified: Tuesday, 8 November 2011, 10:20 AM